Development of Method for Simultaneous Detection of Dibutyl Phthalate and Oleamide in Stingless Bee Honey Harvested from Plastic Cups by Solvent Terminated Dispersive Liquid-liquid Microextraction and Hplc

Recent increased demand for stingless bee honey in Malaysia have stimulated alternative methods to increase honey yields by using plastic cups as artificial honey pots within the beehives. However, honey’s acidity may lead to possible migration of plastic additives, where prolonged consumption may affect body’s physiological responses. This study was carried out to determine the presence of dibutyl phthalate (DBP) and oleamide in stingless bee honey collected from plastic cups. A rapid method for simultaneous extraction and detection of both analytes by Solvent Terminated Dispersive Liquid-Liquid Microextraction (ST-DLLME) coupled with High Performance Liquid Chromatography (HPLC) was developed. ST-DLLME parameters such as type of extraction solvent and disperser solvent were optimized. HPLC parameters optimized to produce good compound separation were Zorbax Eclipse XDB-C18 column, mobile phase acetonitrile: methanol (70:30) and detector wavelength 202nm with total